小鼠小胶质细胞产品信息

培养条件

小胶质细胞培养基
气相：空气，95%；二氧化碳，5%
温度：37摄氏度

细胞描述

细胞生长：贴壁生长
细胞形态：椭圆形或多角形
细胞数量：1×10⁶个
细胞传代：1:3传代；2~3天换液1次
细胞特性：Microglia, one of the glial cell types in the CNS, is an important integral component of the neuro-glial cell network [1]. They have been observed in the brain parenchyma from the early stage of development to the mature state. Microglia act as brain macrophages when programmed cell death occurs during brain development or when the CNS is injured or pathologically damaged. Microglia can be considered the main cell in brain immune surveillance, can present antigens in the molecular context of MHC class II expression to CD-4 positive T cells, are capable of Fc-mediated phagocytosis, and share many common antigens with hemopoietic and tissue macrophages [2]. Furthermore, there is accumulating evidence that microglia are involved in a variety of physiological and pathological processes in the brain by interacting with neurons and other glial cells and through production of biologically active substances such as growth factors, cytokines, and other factors [3]. MM from ScienCell Research Laboratories are isolated from neonate day two mouse brain tissue. Cells are harvested after purification and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. MM is characterized by immunofluorescent method with antibody to OX-42 (CD 11b/c). MM is negative mycoplasma, bacteria, yeast and fungi. MM is guaranteed to further culture in the conditions provided by ScienCell Research Laboratories.
细胞纯度：93％
细胞活力：87％（Viability by Trypan Blue Exclusion）
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌
细胞冻存：液氮冻存（基础培养基+10%DMSO+20%FBS）
细胞运输：干冰运输（1 Vial）或活细胞运输（T-25 flasks）
细胞用途：只可用于科研，不可用于临床诊断和治疗

Reference

[1] Lee, S. C., Liu, W., Brosnan, C. F. and Dickson, D. W. (1992) Characterization of primary human fetal dissociated central nervous system cultures with an emphasis on microgia. Laboratory Investigation. 67:465-476.
[2] Fedoroff, S., Zhai, R. and Novak, J. P. (1997) Microglia and astroglia have a common progenitor cell. J. Neurosci. Res. 50: 477-486.
[3] Stoll, G. and Jander, S. (1999) The role of microglia and macrophages in the pathophysiology of the CNS. Prog. Neurobiol. 58:233-247.

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