小鼠海马神经元细胞产品信息

培养条件

培养基：DMEM/F12+2%B27 或者Neural basal+2%B27
气相：空气，95%；二氧化碳，5%
温度：37℃

细胞描述

本公司培养出品的海马神经元细胞取自新生1天C57小鼠的海马组织，用DMEM/F12+10%FBS完全培养基进行原代培养。

细胞生长：贴壁生长
细胞形态：神经细胞样
细胞数量：1×10⁶个
细胞代数：原代
细胞传代：1:3传代；2~3天换液1次
细胞特性：The tissue of the central nervous system is made up of two classes of cells that may be broadly categorized as neurons and glia. Neurons are anatomic, functional, and trophic units of the brain. Despite great variabilty in size and shape, all neurons share common morphologic features, which are those of the key elements of a highly complex communication network. The neurons are the dynamically polarized cells that serve as the major signaling unit of the nervous system.

The MN-h from ScienCell Research Laboratories are isolated from E18 mouse brain hippocampal tissue. MN-h are cryopreserved at primary cultures and delivered frozen. Each vial contains >1 x 10⁶ cells in 1 ml volume. MN-h are characterized by immunofluorescent method with antibodies to neurafilament, MAP2, and beta-tubulin III. MN-h are negative for mycoplasma, bacteria, yeast and fungi. MN-h are guaranteed to further culture in the conditions provided by ScienCell Research Laboratories.
细胞纯度：93％
细胞活力：87％（Viability by Trypan Blue Exclusion）
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌
细胞冻存：液氮冻存（基础培养基+10%DMSO+20%FBS）
细胞运输：干冰运输（1 Vial）或活细胞运输（T-25 flasks）
细胞用途：只可用于科研，不可用于临床诊断和治疗

产品说明书

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